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BARCELONA PHAGOCYTE WORKSHOP

 

Welcome to the 2001 PHAGOCYTE WORKSHOP

Barcelona, May 2- 5, 2001

    Organisers

 

Dr. Jordi Barquinero

 

 

Departament de Terapia Cel.lular
Institut de Recerca Oncologica
E-08907 L'Hospitalet  (Barcelona), SPAIN
tel.: 34 932633817; fax: 34 932607776

Dr. Faustino Mollinedo

 

 

Centro de Investigación del Cáncer
Instituto de Biología Molecular y Celular del Cáncer
CSIC-Universidad de Salamanca
Campus Miguel de Unamuno
E-37007 Salamanca, SPAIN
tel.: 34 923294806; fax: 34 923294795


 

speakers

programme

posters

 

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    Invited Speakers

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Programme

 

WORKSHOP 12                  Room 6                                       

PHAGOCYTES: FROM BENCH TO CLINICS

 

                                                      Thursday, May 3rd , 2001  

 

Session I        Phagocyte development and apoptosis

Chairmen: Antonio Celada and Steven W. Edwards

 

                                                                                                                             Abstract #

 

9.45-10.15                               Antonio Celada. Barcelona, Spain

Mechanisms of macrophage apoptosis

 

10.15-10.45                           Steven W. Edwards. Liverpool, UK

Molecular control of neutrophil apoptosis

 

10.45-11.00               Coffee break

 

11.00-11.15                V. Witko-Sarsat, S. Canteloup, S. Durant and

B. Descamps-Latscha. Paris, France

Proteinase 3 promotes cell proliferation via the                    189

posttranscriptional regulation of the cyclin-dependent

kinase inhibitor p21/waf1

 

11.15-11.30                S. Sulzbacher, R. Andreesen and M. Rehli.

Regensburg, Germany

The macrophage-specific transcription factor                       191

MTFEC can function as a transcriptional activator

 

11.30-11.45                J. Fritsche, A. Müller, R. Andreesen and

M. Kreutz. Regensburg, Germany

Regulation of ADAM 10 and ADAM 17                               193

expression during human monocyte differentiation

in vitro

 

 

Session II       Recruitment and adhesion of phagocytes

Chairmen: Silvano Sozzani and Peter Hordijk

 

11.45-12.15                Silvano Sozzani. Milan, Italy

Chemokine receptor expression and coupling in regulation of recruitment

 

12.15-12.45                Peter Hordijk. Amsterdam, The Netherlands

Molecular aspects of leukocyte-endothelial interaction and transmigration

 

 

 

 

Session III     Phagocyte exocytosis

 

                                   Chairmen: Manfred Lindau and Faustino Mollinedo

 

12.45-13.15                M. Lindau. Ithaca, NY, USA

Mechanisms of granule tethering and fusion during

eosinophil degranulation

 

13.15-13.30                F. Mollinedo. Salamanca, Spain

SNAREs in neutrophil exocytosis

 

13.30-13.45                M. Lindmark, A. Karlsson, P Francois, L Serrander,

B. Rasmusson, D. Lew, O. Stendahl and O. Nuesse.

Linköping, Sweden

Synaptotagmin II is involved in phagocytosis                        198

and secretion in human neutrophils

 

13.45-14.00                C. Astarie-Dequeker and I. Maridonneaus-Parini.

Toulouse, France

The protein tyrosine kinase Hck as a marker of a                  148

subset of lysosomes in human macrophages

 

 

Session IV     Phagocyte signaling

 

Chairmen: Lodewijk V. Dekker and Mathias P. Wymann

 

16.00-16.30                V. Dekker. London, UK

Protein kinase C isotype signalling in neutrophils

 

16.30-17.00                M.P. Wymann. Fribourg, Switzerland

The G protein-coupled phosphoinositide

3-kinase g- a key to chronic inflammation?

 

17.00-17.15                Per-Arne Oldenborg, H.T. Gresham and F.P.

Lindberg. Umea, Sweden

CD47-SIRPa interaction regulates Fcg and                           180

complement receptor-mediated phagocytosis

 

17.15-17.30                V. Le Cabec, C. Bordier and I. Maridonneau-Parini.

Toulouse, France

Two distinct molecular mechanisms are involved in            188

opsonic and non opsonic phagocytosis mediated by

CR3 (CD11b/CD18)

 

17.30-18.00                Poster session

 

 

 

                                                                  Friday, May 4th, 2001

 

 

Session IV     Phagocyte signaling

 

Chairmen: Lodewijk V. Dekker and Mathias P. Wymann

 

                                                                            Abstract  #

 

09.45-10.00                G. López-Lluch, M.M. Bird, B. Canas,

A.W. Segal and L.V. Dekker. London, U.K.

The C2 domain of PKC-d binds to b-actin and enables        196

PKC-d induced actin redistribution in neutrophils

 

10.00-10.15                           Z. Shmelzer, I. Hazan-Halevy and R. Levy.

Beer Sheva, Israel

Activation of tyrosine kinase PYK2 in neutrophil-like          190

cells stimulated by opsonized zymosan

 

10.15-10.30                           R. Levy, I. Liberty, I. Pesach, N. Haddad, L. Richel,

R. Dana and F. Schlaeffer. Beer Sheva, Israel

Cytosolic phospholipase A2 is essential for                         181

prostaglandin but not for leukotriene formation in

phagocytic-like cells

 

10.30-10.45                           V. Haehnel, M.J. Fenton and M. Rehli.                                 192

Regensburg, Germany

Structure and regulation of the human toll-like

receptor 2 gene in myeloid cells

 

10.45-11.15                           Coffee break

 

 

Session V       NADPH oxidase. Basic and clinical aspects.

 

Chairmen: Anthony W. Segal and Dirk Roos

 

11.15-11.45                           A.W. Segal. London, U.K.

What is the role of superoxide in the phagosome?

 

11.45-12.15                           D. Roos. Amsterdam, The Netherlands

Dysfunctions of the NADPH oxidase

 

12.15-12.30                           S. Chollet-Martin, J. El Benna, M.A.

Gougerot-Pocidalo, B. Grandchamps and B. Gerard.

Paris, France

Characterization of ten novel mutations in the                       182

X-linked chronic granulomatous disease (CYBB gene)

 

12.30-12.45                           P.M.C. Dang, A. Fontayne, J. Hakim, J. El Benna                

and A. Périanin. Paris, France

Protein kinase C z phosphorylates the NADPH                    152

oxidase component, p47phox, on selective sites and

participates in formyl-peptide-mediated neutrophil

respiratory burst

 

12.45-13.00                N. Alloul, Y. Gorzalczany, M. Itan, N. Sigal and

E. Pick. Tel Aviv, Israel

Activation of NADPH oxidase by chimaeric proteins           177

consisting of functional domains of p67phox and Rac1

 

13.00-13.15                 M. Donini, P. Mazzi, D. Lissandrini and S. Dusi.

Verona, Italy

Mechanisms of NADPH oxidase expression in                     171

cultured monocytes

 

13.15-13.30                           D. Shao, R.W. Stockley, L.V. Dekker and A.W.

Segal. London, U.K.

Bundling of actin by the PX domain of p40phox                      151

and p47phox

 

13.30-13.45                           E.P. Reeves, H. Lu, C. Messina, G. Gabella

and A.W. Segal. London, U.K.

An alternative role for superoxide in neutrophils                  155

 

 

                                                                   Saturday, May 5th, 2001

 

 

Session VI     Phagocyte disorders and gene therapy

 

Chairmen: Harry Malech and Manuel Grez

 

                                                                            Abstract #

 

09.45-10.15                Harry Malech. Bethesda, MD, USA

Gene therapy for CGD and other immune deficiencies: novel vectors and approaches

 

10.15-10.45                           Manuel Grez. Frankfurt, Germany

Somatic gene therapy of chronic granulomatous disease

 

10.45-11.15                Coffee break

 

11.15-11.30                           M.J. Stasia, C. Bost-Bru, C. Martel, N. Philippe,

M. Bost and F. Morel. Lyon, France

Association of a nonsense mutation and a splicing                175

error in the CYBB gene responsible for chronic

granulomatous disease (CGD X910)

 

11.30-11.45                           M. Harbord, M. Novelli, A. Hankin, J. Roes and

A.W. Segal. London, U.K.

YM1 crystals in chronic granulomatous disease                    174

mice

 

11.45-12.00                C.G.M. Messina and A.W. Segal. London, U.K.

Catalase negative Staph. aureus are not killed                       150

better than catalase positive strain by murine

p47phox-/- cells

 

12.00-12.15                           J. Bylund, T. Christophe, F. Boulay, T. Nyström,

A. Karlsson and C. Dahlgren. Göteborg, Sweden

Proinflammatory activity of a cecropin-like                          167

antibacterial peptide from Helicobacter pylori

 

12.15-12.30                           I. Hazan-Hallevy, T. Wolak, I. Lubarsky, E. Paran

and R. Levy Beer Sheva, Israel

NADPH-oxidase and phospholipase A2 activities are          165

activated by angiotensin II in neutrophils, monocytes

and phagocytic-like cells

 

12.30-12.45                           M.G. Rittig, M.T. Alvarez-Martinez, F. Porte,

V. Foulongne and B. Rouot. Nottingham, U.K.

Bacteria-induced homotypic fusion of                                   173

Brucella-bearing phagosomes in human monocytes

 

12.45-13.00                A. Alvarez, C. Sanz, A. Pereira, P. Marín and

J. Pétriz. Barcelona, Spain

A flow cytometry-based approach to study human                142

neutrophil function in whole blood

 

16.00-17.30                           Discussion: Gene therapy

Chair: H. Malech, M. Grez.

 

17:30                          End of workshop 12

 

 


 

Workshop 12                                                                                                      Posters

 

 

                                                                                                                      Abstract No

 

NATURAL KILLER-CELL INHIBITION AND APOPTOSIS INDUCED BY                                   143

MONOCYTE-DERIVED RADICALS: ROLE OF MYELOPEROXIDASE

Ĺ. Betten, S. Hermodsson, C.Dahlgren and K.Hellstrand. Göteborg, Sweden

 

EXPRESSION OF APOPTOSIS-RELATED GENES IN HUMAN NEUTROPHILS                        144

AND NEUTROPHIL-DIFFERENTIATING HL-60 CELLS.

A. M. Santos-Beneit and F Mollinedo. Salamanca, Spain.

 

Galectin-1 activation of the neutrophil RESPIRATORY burst                             145

K. Almkvist, C. Dahlgren, H. Leffler and A. Karlsson. Göteborg and Lund, Sweden.

 

IDENTIFICATION AND CHARACTERIZATION OF SUPEROXIDE                                               146

GENERATING NADPH OXIDASE IN THE CELLULAR SLIME MOLD

DICTYOSTELIUM DISCOIDEUM

B. Lardy ,M.H. Paclet, M. Bof, F. Morel, M. Satre, and G. Klein. Grenoble, France.

 

SNAP-23, SYNTAXIN 6 AND SYNAPTOTAGMIN IN NEUTROPHIL EXOCYTOSIS                 147

B. Martín-Martín, S.M. Nabokina, J. Blasi, P.A. Lazo and F. Mollinedo.

Salamanca, Spain.

 

INTRACELLULAR POOL OF IL-10 RECEPTORS IN SPECIFIC GRANULES                             149

OF HUMAN NEUTROPHILS. DIFFERENTIAL MOBILISATION BY

PROINFLAMMATORY MEDIATORS

C. Elbim, H. Réglier, M. Fay, C. Delarche, V. Andrieu, J. El Benna, M.A.

Gougerot-Pocidalo. Paris, France.

 

Hyperglycemia inhibits phagocytosis of C3bi- and IgG-opsonized                   153

yeast particles in normal human neutrophils: Role of Protein

Kinase C (PKC) activation

D. Saiepour, J. Sehlin and P.A. Oldenborg. Umeĺ, Sweden.

 

Activation of NADPH oxidase and p47phox phosphorylation: Role               154

of ERK1/2 and p38 mitogen activated kinases

C. Dewas., M-A. Gougerot-Pocidalo and J. El-Benna. Paris, France.

 

HUMAN P40-PHOX GENE REGULATION IN MONOCYTIC CELLS.                                          156

E. Conte, L. Manzella, G. Cocchiaro, L. Messina and A. Messina . Catania, Italy.

 

INTERLEUKIN 12 INCREASES IL-8 PRODUCTION AND RELEASE BY HUMAN                   157

POLYMORPHONUCLEAR NEUTROPHILS (PMN) VIA TNF-a AND IFN-g

F. Ethuin, C. Delarche, S. Benslama, M-A. Gougerot-Pocidalo, L. Jacob, and

S. Chollet-Martin. Paris, France.

                                                                                                                                                                 

ATORVASTATIN IS THE MOST POTENT HMG-CoA REDUCTASE INHIBITOR                        158

INHIBITING HUMAN PHAGOCYTE Fcg RECEPTOR EXPRESSION

F. Gómez, P. Ruiz, J.A. Bernal, S. Romero, R. López and C. Rivera. Cádiz, Spain.

 

PHYSICAL PARAMETERS OF THE INTERACTIONS                                                                      159

BETWEEN THE CYTOSOLIC phox PROTEINS OF THE

HUMAN NEUTROPHIL NADPH OXIDASE

F.B. Wientjes, R.R.J. O’Brien, J.E. Ladbury and A.W. Segal. London, UK.

 

DIFFERENT FUNCTIONAL ROLES OF Fc RECEPTORS FOR IgG ON                                        160

NEUTROPHILS.

G. Fossati, R.C. Bucknall and S. W. Edwards. Liverpool, UK.

 

INHIBITION OF LEUKOCYTES LECTIN-STIMULATED AGGREGATION                                  161

BY ANTITHROMBIN

G. Ricevuti, D. Gritti, A. Malinverno, C. Muselli, J. Romisch* and C.J. Wiedermann§

Dept. of Internal Medicine and Therapeutics, IRCCS S. Matteo, University of Pavia, Italy,

*Avensis Behring, Marburg, Germany, § Dept. Internal Medicine, University of Innsbruck, Austria.

 

Effect of advanced glycosylation end products on superoxide                 162

Generation by human PMNs

R. Gavrieli, G. Rashid,  J. Bernheim and B. Wolach.

Dept. of Nephrology and Pediatrics, The Pediatric Hematology Clinic and the Laboratory

for Leukocyte Function, Sapir Medical Center, Kfar Sava, Tel-Aviv University, Israel.

 

AN INTACT CYTOSKELETON IS REQUIRED FOR PROLONGED RESPIRATORY                   163

BURST ACTIVITY DURING NEUTROPHIL PHAGOCYTOSIS.

D. Granfeldt and C. Dahlgren. Göteborg, Sweden

 

THE CHARGE GENERATED BY THE NADPH OXIDASE OF HUMAN NEUTROPHIL             164

COMPENSATED PARTIALLY BY POTASSIUM.

H. Lu, E. P. Reeves, G. Gabella, A.W. Segal. London, UK

 

ESSENTIAL REQUIREMENT OF CYTOSOLIC PHOSPHOLIPASE A2 FOR                                 166

STIMULATION OF NADPH OXIDASE-ASSOCIATED DIAPHORASE ACTIVITY

IN GRANULOCYTE-CELL LINES.

I. Pessach, T.L. Leto, H.L. Malech, R. Levy. Beer Sheva, Israel. Bethesda, USA .

 

THE REGULATORY ROLE OF ADP-RIBOSYLATION FACTOR AND                                          168       

PHOSPHOLIPASE D IN THE ACTIVATION OF NEUTROPHIL GRANULOCYTES.

K.Káldi, J. Szeberényi, M. Geiszt, A. Mócsai and E. Ligeti. Budapest, Hungary.

 

MOLECULAR BASES OF HUMAN NEUTROPHIL RESPONSIVENESS TO                                169

Interleukin-10.

L. Crepaldi, S. Gasperini, J.A. Lapinet, C. Pinardi and M.A. Cassatella. Verona, Italy.

 

STREPTOCOCCUS PYOGENES ARE EFFICIENTLY PHAGOCYTOSED BY                              170

HUMAN NEUTROPHILS: EVIDENCE FOR INTRACELLULAR SURVIVAL

OF BACTERIA.

L. Staali, L. Björck and H. Tapper. Lund, Sweden.

 

PROTEOMICS APPROACH TO IDENTIFY PROTEINS PHOSPHORYLATED                             172

IN FMLP-STIMULATED NEUTROPHILS

M.H. Paclet, A.W. Segal and L.V. Dekker. London , UK.

 

MELATONIN OXIDATION IN MACROPHAGES                                                                             176

M.R. Rodrigues, D. Rodriguez, L.H. Catalani, M. Russo, A. Campa. Săo Paulo, Brazil

 

SECRETED PHOSPHOLIPASE A2 TYPE V IS ACTIVATED AND RELEASED                            178

BY PERIPHERAL GRANULOCYTES AND DIFFERENTIATED PLB-985 CELLS

N. Hadad, Y. Svets and R. Levy. Beer Sheva, Israel.

 

IN VITRO BIOGENESIS OF PHAGOLYSOSOMES IS CONTROLLED BY                                   179       

MYCOBACTERIA AND HCK.

P. Peyron, C. Astarie-Dequeker, C. Bordier, T. Stegmann and

I. Maridonneau-Parini. Toulouse Cedex

 

ATP-DEPENDENCE OF NEUTROPHIL EXOCYTOSIS                                                                   183

S. Theander1, D.P. Lew1, O. Nüsse2

(1) Geneva, Switzerland. (2) Vandoeuvre, France

 

CLINICAL HETEROGENEITY OF PHAGOCYTIC DISORDERS                                                    186

T. Espańol, I.Caragol, C.Figueras and JJ.Ortega. Barcelona. Spain

 

MYELOABLATIVE TREATMENT ENHANCES THE ENGRAFTMENT OF                                   187

TRANSDUCED MURINE HEMATOPOIETIC CELLS IN A DOSE-DEPENDENT

MANNER.

T. Puig, E. Kádár, A. Limón, J.A. Cancelas, M. García, H. Eixarch, L. Luquín and

J. Barquinero. L’Hospitalet. Spain.

 

Decysin is regulated By 1a,25-Dihydroxy-vitamin D3 during the                      194

Differentiation of human monocytes

J. Fritsche, A. Müller, R. Andreesen and M. Kreutz. Regensburg, Germany.

 

PROTEIN KINASE C ISOTYPE FUNCTION IN NEUTROPHILS                                                    197

L.V. Dekker, J.K. Davidson-Moncada, G. Lopez-Lluch, M. Leitges, M.H. Paclet, N. Wong

and A.W. Segal. London, UK.

 

 

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    Congress location and city information

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  Sponsors

        CGD Research Trust       

 

BD Biociencias Espańa:

BD Biosciences               BD Labware            BD Immunocytometry Systems

BD Transduction Labs    BD Pharmingen        BD Clontech
 

IZASA


Further Information:
   Inquiries about this Phagocyte Workshop may be addressed to:
   Jordi Barquinero (jbarquinero@iro.es) or Faustino Mollinedo (fmollin@usal.es)


 

speakers

programme

posters

location

sponsors