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Dr. Jordi Barquinero
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Dr. Faustino Mollinedo
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Chairmen: Antonio Celada and Steven W. Edwards
Abstract
#
9.45-10.15
Antonio
Celada. Barcelona, Spain
10.15-10.45
Steven
W. Edwards. Liverpool,
UK
10.45-11.00 Coffee
break
11.00-11.15 V.
Witko-Sarsat, S. Canteloup, S. Durant and
B. Descamps-Latscha. Paris, France
Proteinase 3 promotes cell
proliferation via the 189
posttranscriptional
regulation of the cyclin-dependent
kinase inhibitor p21/waf1
11.15-11.30 S.
Sulzbacher, R. Andreesen and M. Rehli.
Regensburg, Germany
The macrophage-specific
transcription factor 191
MTFEC can function as a
transcriptional activator
11.30-11.45 J.
Fritsche, A. Müller, R. Andreesen and
M. Kreutz. Regensburg, Germany
Regulation of ADAM 10 and ADAM 17 193
expression during human monocyte differentiation
in
vitro
Chairmen: Silvano Sozzani and Peter Hordijk
11.45-12.15 Silvano Sozzani. Milan,
Italy
Chemokine receptor
expression and coupling in regulation of recruitment
12.15-12.45 Peter
Hordijk. Amsterdam, The Netherlands
Molecular aspects of
leukocyte-endothelial interaction and transmigration
Session
III Phagocyte
exocytosis
Chairmen: Manfred Lindau and
Faustino Mollinedo
12.45-13.15 M. Lindau. Ithaca, NY, USA
Mechanisms of granule tethering and
fusion during
eosinophil degranulation
13.15-13.30 F.
Mollinedo. Salamanca, Spain
SNAREs in neutrophil exocytosis
13.30-13.45 M.
Lindmark, A. Karlsson, P Francois, L Serrander,
B. Rasmusson, D. Lew, O. Stendahl and O. Nuesse.
Linköping, Sweden
Synaptotagmin II is involved in
phagocytosis 198
and secretion in human
neutrophils
13.45-14.00 C.
Astarie-Dequeker and I. Maridonneaus-Parini.
Toulouse, France
The protein tyrosine kinase Hck as a
marker of a 148
subset of lysosomes in human macrophages
Chairmen: Lodewijk V. Dekker and Mathias P. Wymann
16.00-16.30 V.
Dekker. London, UK
16.30-17.00
M.P. Wymann. Fribourg,
Switzerland
The G protein-coupled
phosphoinositide
3-kinase g- a key to chronic inflammation?
17.00-17.15 Per-Arne
Oldenborg, H.T. Gresham and F.P.
Lindberg. Umea, Sweden
CD47-SIRPa interaction regulates Fcg and 180
complement
receptor-mediated phagocytosis
17.15-17.30 V.
Le Cabec, C. Bordier and I. Maridonneau-Parini.
Toulouse, France
Two distinct molecular mechanisms
are involved in 188
opsonic and non opsonic
phagocytosis mediated by
CR3 (CD11b/CD18)
17.30-18.00 Poster
session
Chairmen: Lodewijk V. Dekker and Mathias P. Wymann
Abstract #
09.45-10.00 G.
López-Lluch, M.M. Bird, B. Canas,
A.W. Segal and L.V. Dekker. London, U.K.
The C2 domain of PKC-d binds to b-actin and enables 196
PKC-d induced actin redistribution in neutrophils
10.00-10.15
Z.
Shmelzer, I. Hazan-Halevy and R. Levy.
Beer Sheva, Israel
Activation of tyrosine kinase
PYK2 in neutrophil-like 190
cells stimulated by opsonized
zymosan
10.15-10.30
R.
Levy, I. Liberty, I. Pesach, N. Haddad, L. Richel,
R. Dana and F. Schlaeffer. Beer
Sheva, Israel
Cytosolic phospholipase A2 is essential for 181
prostaglandin but not for leukotriene formation
in
phagocytic-like cells
10.30-10.45
V.
Haehnel, M.J. Fenton and M. Rehli. 192
Regensburg, Germany
Structure and regulation of
the human toll-like
receptor 2 gene in myeloid
cells
10.45-11.15
Coffee
break
11.15-11.45
A.W.
Segal. London, U.K.
11.45-12.15
D.
Roos. Amsterdam, The Netherlands
12.15-12.30
S.
Chollet-Martin, J. El Benna, M.A.
Gougerot-Pocidalo, B. Grandchamps
and B. Gerard.
Paris, France
Characterization of ten
novel mutations in the 182
X-linked chronic
granulomatous disease (CYBB gene)
12.30-12.45
P.M.C.
Dang, A. Fontayne, J. Hakim, J. El Benna
and A. Périanin. Paris, France
Protein kinase C z phosphorylates the NADPH 152
oxidase component, p47phox, on
selective sites and
participates in formyl-peptide-mediated
neutrophil
respiratory burst
12.45-13.00 N.
Alloul, Y. Gorzalczany, M. Itan, N. Sigal and
E.
Pick. Tel Aviv, Israel
Activation of NADPH oxidase by chimaeric
proteins 177
consisting of functional domains of p67phox
and Rac1
13.00-13.15
M. Donini,
P. Mazzi, D. Lissandrini and S. Dusi.
Verona, Italy
Mechanisms of NADPH oxidase expression in 171
cultured monocytes
13.15-13.30
D. Shao, R.W. Stockley,
L.V. Dekker and A.W.
Segal. London, U.K.
Bundling of actin by the PX
domain of p40phox 151
and p47phox
13.30-13.45
E.P. Reeves, H. Lu, C.
Messina, G. Gabella
and A.W. Segal. London, U.K.
An alternative role for
superoxide in neutrophils 155
Saturday, May 5th, 2001
Abstract #
09.45-10.15 Harry
Malech. Bethesda, MD, USA
Gene therapy for CGD and other immune deficiencies:
novel vectors and approaches
10.15-10.45
Manuel Grez. Frankfurt, Germany
Somatic gene therapy of chronic granulomatous disease
10.45-11.15 Coffee
break
11.15-11.30
M.J. Stasia, C. Bost-Bru, C. Martel, N. Philippe,
M.
Bost and F. Morel. Lyon, France
Association of a nonsense mutation and a splicing 175
error in the CYBB gene responsible for chronic
granulomatous disease (CGD X910)
11.30-11.45
M. Harbord, M. Novelli, A. Hankin, J. Roes and
A.W.
Segal. London, U.K.
YM1 crystals in chronic granulomatous disease 174
mice
11.45-12.00 C.G.M.
Messina and A.W. Segal. London, U.K.
Catalase negative Staph. aureus are not killed 150
better than catalase positive strain by murine
p47phox-/- cells
12.00-12.15
J. Bylund, T. Christophe, F. Boulay, T. Nyström,
A.
Karlsson and C. Dahlgren. Göteborg, Sweden
Proinflammatory activity of a cecropin-like 167
antibacterial peptide from Helicobacter pylori
12.15-12.30
I. Hazan-Hallevy, T. Wolak, I. Lubarsky, E. Paran
and R.
Levy Beer Sheva, Israel
NADPH-oxidase and phospholipase A2 activities are 165
activated by angiotensin II in neutrophils, monocytes
and phagocytic-like cells
12.30-12.45
M.G.
Rittig, M.T. Alvarez-Martinez, F. Porte,
V.
Foulongne and B. Rouot. Nottingham, U.K.
Bacteria-induced homotypic fusion of 173
Brucella-bearing phagosomes in human monocytes
12.45-13.00 A.
Alvarez, C. Sanz, A. Pereira, P. Marín and
J. Pétriz. Barcelona, Spain
A flow cytometry-based approach to study human 142
neutrophil function in whole blood
16.00-17.30
Discussion: Gene therapy
Chair: H. Malech, M. Grez.
17:30 End of workshop 12
Workshop 12 Posters
NATURAL KILLER-CELL INHIBITION
AND APOPTOSIS INDUCED BY 143
MONOCYTE-DERIVED RADICALS: ROLE
OF MYELOPEROXIDASE
Ĺ. Betten, S. Hermodsson,
C.Dahlgren and K.Hellstrand. Göteborg, Sweden
EXPRESSION
OF APOPTOSIS-RELATED GENES IN HUMAN NEUTROPHILS 144
AND
NEUTROPHIL-DIFFERENTIATING HL-60 CELLS.
A. M. Santos-Beneit
and F Mollinedo. Salamanca, Spain.
Galectin-1 activation of the
neutrophil RESPIRATORY burst 145
K. Almkvist, C. Dahlgren, H. Leffler and A.
Karlsson. Göteborg and Lund, Sweden.
IDENTIFICATION AND CHARACTERIZATION OF
SUPEROXIDE 146
GENERATING NADPH OXIDASE IN THE CELLULAR SLIME
MOLD
DICTYOSTELIUM DISCOIDEUM
B. Lardy ,M.H. Paclet, M. Bof, F. Morel, M.
Satre, and G. Klein. Grenoble, France.
SNAP-23,
SYNTAXIN 6 AND SYNAPTOTAGMIN IN NEUTROPHIL EXOCYTOSIS 147
B. Martín-Martín, S.M. Nabokina, J.
Blasi, P.A. Lazo and F. Mollinedo.
Salamanca,
Spain.
INTRACELLULAR POOL OF IL-10 RECEPTORS IN SPECIFIC
GRANULES 149
OF HUMAN NEUTROPHILS. DIFFERENTIAL MOBILISATION BY
PROINFLAMMATORY MEDIATORS
C. Elbim, H.
Réglier, M. Fay, C. Delarche, V. Andrieu, J. El Benna, M.A.
Gougerot-Pocidalo.
Paris, France.
Hyperglycemia inhibits phagocytosis of C3bi- and IgG-opsonized 153
yeast particles in normal human neutrophils: Role of
Protein
Kinase C (PKC) activation
D.
Saiepour, J. Sehlin and P.A. Oldenborg. Umeĺ, Sweden.
Activation
of NADPH oxidase and p47phox phosphorylation: Role 154
of
ERK1/2 and p38 mitogen activated kinases
C. Dewas., M-A. Gougerot-Pocidalo
and J. El-Benna. Paris, France.
HUMAN
P40-PHOX GENE REGULATION IN MONOCYTIC CELLS. 156
E.
Conte, L. Manzella, G. Cocchiaro, L. Messina and A. Messina . Catania, Italy.
INTERLEUKIN 12 INCREASES
IL-8 PRODUCTION AND RELEASE BY HUMAN 157
POLYMORPHONUCLEAR
NEUTROPHILS (PMN) VIA TNF-a AND IFN-g
F.
Ethuin, C. Delarche, S. Benslama, M-A. Gougerot-Pocidalo, L.
Jacob, and
S. Chollet-Martin.
Paris, France.
ATORVASTATIN IS THE MOST
POTENT HMG-CoA REDUCTASE INHIBITOR 158
INHIBITING HUMAN
PHAGOCYTE Fcg RECEPTOR EXPRESSION
F.
Gómez, P. Ruiz, J.A. Bernal, S. Romero, R. López and C. Rivera. Cádiz, Spain.
PHYSICAL PARAMETERS OF THE INTERACTIONS 159
BETWEEN
THE CYTOSOLIC phox PROTEINS OF THE
HUMAN
NEUTROPHIL NADPH OXIDASE
F.B.
Wientjes, R.R.J. O’Brien, J.E. Ladbury and A.W. Segal. London, UK.
DIFFERENT
FUNCTIONAL ROLES OF Fc RECEPTORS FOR IgG ON 160
NEUTROPHILS.
G.
Fossati, R.C. Bucknall and S. W. Edwards. Liverpool,
UK.
INHIBITION OF LEUKOCYTES LECTIN-STIMULATED
AGGREGATION 161
BY ANTITHROMBIN
G. Ricevuti, D. Gritti, A. Malinverno, C.
Muselli, J. Romisch* and C.J. Wiedermann§
Dept. of Internal Medicine and Therapeutics, IRCCS S. Matteo, University
of Pavia, Italy,
*Avensis Behring, Marburg, Germany, § Dept. Internal Medicine,
University of Innsbruck, Austria.
Effect of
advanced glycosylation end products on superoxide 162
Generation
by human PMNs
R. Gavrieli, G. Rashid, J. Bernheim and B. Wolach.
Dept. of Nephrology and
Pediatrics, The Pediatric Hematology Clinic and the Laboratory
for Leukocyte Function, Sapir
Medical Center, Kfar Sava, Tel-Aviv University, Israel.
AN INTACT CYTOSKELETON IS REQUIRED FOR
PROLONGED RESPIRATORY 163
BURST ACTIVITY DURING NEUTROPHIL PHAGOCYTOSIS.
D. Granfeldt and C. Dahlgren. Göteborg, Sweden
THE CHARGE GENERATED BY THE NADPH
OXIDASE OF HUMAN NEUTROPHIL 164
COMPENSATED PARTIALLY BY
POTASSIUM.
H. Lu, E. P. Reeves, G. Gabella, A.W. Segal. London, UK
ESSENTIAL REQUIREMENT OF CYTOSOLIC PHOSPHOLIPASE A2 FOR 166
STIMULATION OF NADPH OXIDASE-ASSOCIATED DIAPHORASE ACTIVITY
IN GRANULOCYTE-CELL LINES.
THE REGULATORY ROLE OF
ADP-RIBOSYLATION FACTOR AND 168
PHOSPHOLIPASE D IN THE
ACTIVATION OF NEUTROPHIL GRANULOCYTES.
K.Káldi, J. Szeberényi, M. Geiszt, A. Mócsai and E.
Ligeti. Budapest, Hungary.
MOLECULAR BASES OF HUMAN
NEUTROPHIL RESPONSIVENESS TO 169
Interleukin-10.
L. Crepaldi, S. Gasperini, J.A. Lapinet, C. Pinardi
and M.A. Cassatella. Verona, Italy.
STREPTOCOCCUS
PYOGENES ARE EFFICIENTLY PHAGOCYTOSED BY 170
HUMAN NEUTROPHILS:
EVIDENCE FOR INTRACELLULAR SURVIVAL
OF BACTERIA.
L. Staali, L. Björck and H. Tapper. Lund,
Sweden.
PROTEOMICS APPROACH TO IDENTIFY PROTEINS
PHOSPHORYLATED 172
IN FMLP-STIMULATED NEUTROPHILS
M.H. Paclet, A.W. Segal and L.V. Dekker. London ,
UK.
MELATONIN
OXIDATION IN MACROPHAGES 176
M.R. Rodrigues, D. Rodriguez, L.H. Catalani, M.
Russo, A. Campa. Săo Paulo,
Brazil
SECRETED PHOSPHOLIPASE A2 TYPE V IS ACTIVATED AND RELEASED 178
BY PERIPHERAL GRANULOCYTES AND DIFFERENTIATED
PLB-985 CELLS
N. Hadad, Y. Svets and R.
Levy. Beer Sheva, Israel.
IN VITRO BIOGENESIS OF
PHAGOLYSOSOMES IS CONTROLLED BY 179
MYCOBACTERIA AND HCK.
P.
Peyron, C. Astarie-Dequeker, C. Bordier, T. Stegmann and
I. Maridonneau-Parini. Toulouse Cedex
ATP-DEPENDENCE
OF NEUTROPHIL EXOCYTOSIS 183
S.
Theander1, D.P. Lew1, O. Nüsse2
(1)
Geneva, Switzerland. (2) Vandoeuvre, France
CLINICAL HETEROGENEITY OF
PHAGOCYTIC DISORDERS 186
T. Espańol, I.Caragol, C.Figueras
and JJ.Ortega. Barcelona. Spain
MYELOABLATIVE TREATMENT ENHANCES THE
ENGRAFTMENT OF 187
TRANSDUCED MURINE HEMATOPOIETIC CELLS IN A
DOSE-DEPENDENT
MANNER.
T. Puig, E. Kádár, A. Limón, J.A. Cancelas, M. García,
H. Eixarch, L. Luquín and
J. Barquinero. L’Hospitalet. Spain.
Decysin is regulated By 1a,25-Dihydroxy-vitamin
D3 during the 194
Differentiation of human
monocytes
J. Fritsche, A. Müller, R. Andreesen and M. Kreutz.
Regensburg, Germany.
PROTEIN KINASE C ISOTYPE FUNCTION IN NEUTROPHILS 197
L.V. Dekker, J.K. Davidson-Moncada, G. Lopez-Lluch,
M. Leitges, M.H. Paclet, N. Wong
and
A.W. Segal. London, UK.
ˇ How to obtain a registration form
For more information about Barcelona click here
BD Biociencias Espańa:
BD Biosciences
BD Labware BD
Immunocytometry Systems
BD
Transduction Labs BD Pharmingen BD
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Further
Information:
Inquiries about this Phagocyte Workshop may be addressed
to:
Jordi Barquinero (jbarquinero@iro.es) or Faustino Mollinedo (fmollin@usal.es)
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